Bai, F and Branch, R W and Nicolau jr, D. V. and Pilizota, T and Steele, B C and Maini, P. K. and Berry, R M (0210) Conformational spread as a mechanism for cooperativity in the bacterial flagellar switch. Science, 327 (5966). pp. 685-689.
The bacterial flagellar switch that controls the direction of flagellar rotation during chemotaxis has a highly cooperative response. This has previously been understood in terms of the classic two-state, concerted model of allosteric regulation. Here, we used high-resolution optical microscopy to observe switching of single motors and uncover the stochastic multistate nature of the switch. Our observations are in detailed quantitative agreement with a recent general model of allosteric cooperativity that exhibits conformational spread—the stochastic growth and shrinkage of domains of adjacent subunits sharing a particular conformational state. We expect that conformational spread will be important in explaining cooperativity in other large signaling complexes.
|Subjects:||A - C > Biology and other natural sciences|
|Research Groups:||Centre for Mathematical Biology|
|Deposited By:||Philip Maini|
|Deposited On:||09 Feb 2010 07:44|
|Last Modified:||08 Oct 2012 09:41|
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