Lin, C.M and Jiang, T.X. and Baker, Ruth E. and Maini, P. K. and Widelitz, R. B. and Chunong, C.M. (2009) Spots & stripes: pleomorphic patterning of stem cells via p-ERK-depenendent cell chemotaxis shown by feather morphogenesis & mathematical simulation. Developmental Biology , 334 (2). pp. 369-382.
|PDF - Published Version|
A key issue in stem cell biology is the differentiation of homogeneous stem cells towards different fates which are also organized into desired configurations. Little is known about the mechanisms underlying the process of periodic patterning. Feather explants offer a fundamental and testable model in which multi-potential cells are organized into hexagonally arranged primordia and the spacing between primordia. Previous work explored roles of a Turing reaction–diffusion mechanism in establishing chemical patterns. Here we show that a continuum of feather patterns, ranging from stripes to spots, can be obtained when the level of p-ERK activity is adjusted with chemical inhibitors. The patterns are dose-dependent, tissue stage-dependent, and irreversible. Analyses show that ERK activity-dependent mesenchymal cell chemotaxis is essential for converting micro-signaling centers into stable feather primordia. A mathematical model based on short-range activation, long-range inhibition, and cell chemotaxis is developed and shown to simulate observed experimental results. This generic cell behavior model can be applied to model stem cell patterning behavior at large.
|Uncontrolled Keywords:||Pattern formation; Feather morphogenesis; Stem cells; Placode; ERK; Mathematical modeling; Chemotaxis|
|Subjects:||A - C > Biology and other natural sciences|
|Research Groups:||Centre for Mathematical Biology|
|Deposited By:||Philip Maini|
|Deposited On:||01 Oct 2009 07:46|
|Last Modified:||25 Oct 2010 14:29|
Repository Staff Only: item control page