The Mathematical Institute, University of Oxford, Eprints Archive

Use and abuse of the quasi-steady-state approximation

Flach, E. H. and Schnell, S. (2006) Use and abuse of the quasi-steady-state approximation. IEE Proceedings Systems Biology, 153 (4). pp. 187-191.

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Abstract

The transient kinetic behaviour of an open single enzyme, single substrate reaction is examined. The reaction follows the Van Slyke–Cullen mechanism, a spacial case of the Michaelis–Menten reaction. The analysis is performed both with and without applying the quasi-steady-state approximation. The analysis of the full system shows conditions for biochemical pathway coupling, which yield sustained oscillatory behaviour in the enzyme reaction. The reduced model does not demonstrate this behaviour. The results have important implications in the analysis of open biochemical reactions and the modelling of metabolic systems.

Item Type:Article
Additional Information:n/a
Uncontrolled Keywords:quasisteady-state approximation; transient kinetic behaviour; open single enzyme; single substrate reaction; Van Slyke-Cullen mechanism; Michaelis-Menten reaction; biochemical pathway coupling; oscillatory behaviour; metabolic systems
Subjects:A - C > Biology and other natural sciences
Research Groups:Centre for Mathematical Biology
ID Code:591
Deposited By:Philip Maini
Deposited On:16 May 2007
Last Modified:20 Jul 2009 14:22

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