The Mathematical Institute, University of Oxford, Eprints Archive

A century of enzyme kinetics. Should we believe in the Km and vmax estimates?

Schnell, S. and Maini, P. K. (2003) A century of enzyme kinetics. Should we believe in the Km and vmax estimates? Comments in Theoretical Biology, 8 (2-3). pp. 169-187.

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Abstract

The application of the quasi-steady-state approximation (QSSA) in biochemical kinetics allows the reduction of a complex biochemical system with an initial fast transient into a simpler system. The simplified system yields insights into the behavior of the biochemical reaction, and analytical approximations can be obtained to determine its kinetic parameters. However, this process can lead to inaccuracies due to the inappropriate application of the QSSA. Here we present a number of approximate solutions and determine in which regions of the initial enzyme and substrate concentration parameter space they are valid. In particular, this illustrates that experimentalists must be careful to use the correct approximation appropriate to the initial conditions within the parameter space.

Item Type:Article
Uncontrolled Keywords:Enzyme Kinetics, Michaelis-Menten, Quasi-steady-state Approximation, Fitting Procedures, Progress Curves
Subjects:A - C > Biology and other natural sciences
Research Groups:Centre for Mathematical Biology
ID Code:381
Deposited By:Philip Maini
Deposited On:17 Nov 2006
Last Modified:20 Jul 2009 14:20

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