Osborne, J. M. and O'Dea, R. D. and Whiteley, J. P. and Byrne, H. M. and Waters, S. L.
(2010)
The influence of bioreactor geometry and the mechanical
environment on engineered tissues.
ASME Journal of Biomechanical Engineering, 132 No
(pp 510).

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Abstract
A three phase model for the growth of a tissue construct within a perfusion bioreactor is examined. The cell population (and attendant extracellular matrix), culture medium, and porous scaffold are treated as distinct phases. The bioreactor system is represented by a twodimensional channel containing a cellseeded rigid porous scaffold (tissue construct), which is perfused with a culture medium. Through the prescription of appropriate functional forms for cell proliferation and extracellular matrix deposition rates, the model is used to compare the influence of cell density, pressure, and culture medium shear stressregulated growth on the composition of the engineered tissue. The governing equations are derived in O’Dea et al. “A Three Phase Model for Tissue Construct Growth in a Perfusion Bioreactor,” Math. Med. Biol., in which the longwavelength limit was exploited to aid analysis; here, finite element methods are used to construct twodimensional solutions to the governing equations and to investigate thoroughly their behavior. Comparison of the total tissue yield and averaged pressures, velocities, and shear stress demonstrates that quantitative agreement between the twodimensional and longwavelength approximation solutions is obtained for channel aspect ratios of order 10 to the negative 2 and that much of the qualitative behavior of the model is captured in the longwavelength limit, even for relatively large channel aspect ratios. However, we demonstrate that in order to capture accurately the effect of mechanotransduction mechanisms on tissue construct growth, spatial effects in at least two dimensions must be included due to the inherent spatial variation of mechanical stimuli relevant to perfusion bioreactors, most notably, fluid shear stress, a feature not captured in the longwavelength limit.
Item Type:  Article 

Subjects:  D  G > General 
Research Groups:  Oxford Centre for Collaborative Applied Mathematics 
ID Code:  1033 
Deposited By:  Peter Hudston 
Deposited On:  07 Jan 2011 08:46 
Last Modified:  29 May 2015 18:43 
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